Uji Aktivitas Antibakteri dari Ekstrak Etanol dan Beberapa Fraksi Daun Kenikir (Cosmos caudatus Kunth.) terhadap Bakteri Penyebab Disentri Shigella sp

Ema Ratna Sari, Nilda Lely, Dian Septimarleti

Abstract


It has been tested the antibacterial activity of ethanol extract and some fractions of leaves marigolds (Cosmos caudatus Kunth.) againts the bacteria Shigella dysentriae ATCC 13313, Shigella flexneri ATCC 12022 dan Shigella boydii ATCC 12985. Extraction is done by maceration method with a yield 3.62% b/b results of the fractionation was then performed using the solvent n-hexane, ethyl acetate, and water. Chemical content ob-tained in the test phytochemical that leaves marigolds (Cosmos caudatus Kunth.) Are chemical compounds of alkaloids, flavonoids, phenolics, saponins and steroids. Antibacterial activity assay carried out using agar diffu-sion method with 5 variations of concentration of test substance, wich is 10%, 15%, 20%, 25% and 30% with used destilation etanol as negative control and tetracyclin as positive control. The results of inhibition zone di-ameter measurements the ethanol extract and some fractions have a antibacterial activity againts Shigella dy-sentriae ATCC 13313, Shigella flexneri ATCC 12022 and Shigella boydii ATCC 12985 At 30% concentration of test subtance to bacteria Shigella dysentriae ATCC 13313 inhibitory zone diameters on ethanol extract leaves marigolds of 17.0 mm, n-hexane fraction of 13.8 mm, ethyl acetate fraction of 14.3 mm, and water fraction of 10.9 mm. Againts Shigella flexneri ATCC 12022 the diameter of inhibitory zone diameters on ethanol extract leaves marigolds of 18.6 mm, n-hexane fraction of 15.3 mm, ethyl acetate fraction 15.7 mm and water fraction of 13.8 mm. Againts Shigella boydii ATCC 12985 the diameter of inhibitory zone diameters on ethanol extract leaves marigolds of 20.2 mm, n-hexane fraction of 16.1 mm, ethyl acetate fraction of 16.9 mm and water frac-tion of 15.5 mm. So, that has the greatest antibacterial activity shown in the ethanol extract of leaves marigolds to the bacteria Shigella boydii ATCC 12985 at 30 % concentration of the test substance with inhibition zone di-ameter of 20.2 mm.

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References


Abas, F., Shaari, K., Lajis, N.H., Israf, D.A., & Kalsom, Y.U. 2003. Antioxidative and radical scavenging prop-erties of the constituents isolated from (Cosmos cauda-tus Kunth.). Nat. prod. sciences, 9(4), 245-248.

Ahmed, Bahar. 2007. Chemistry of natural products. New Delhi: Departement of Pharmaceutical Chemistry Faculty of Science Jamia Hamdard.

Black, J.M. & Jacobs, E.M. 1993. Medical surgical nurs-ing (4th edition). Philadelphia: W.B. Saunders Compa-ny.

Cappuccino and James G. 2009. Manual laboratorium mikrobiologi. Jakarta: EGC Medical Publisher.

Culvenor, C.C.J & J.S. Fitzgerald. 1963. A field me-thod for alkaloid screening of plant. J. Pharm Sci. 52, 303-304.

Djamal, R. 2010. Kimia Bahan Alam:Prinsip-prinsip dasar isolasi dan identifikasi. Padang: Penerbit Universitas Baiturrahmah.

Dwidjoseputro, D. 1998. Dasar-dasar mikrobiologi. Jakarta: Djambatan.

Gupte, Satis. 1990. Mikrobiologi dasar (Edisi III). Jakar-ta: Bina Aksara.

Harbone, J.B. 1987. Metode fitokimia penuntun cara modern menganalisa tumbuhan. Diterjemahkan oleh K. Padmawinata. Bandung: ITB Press.

Harmita & Radji, M. 2008. Buku ajar analisis hayati. Jakarta : EGC.

Jawetz, E., Melnick, J.L & E. A. Adelberg. 2005. Mi-krobiologi kedokteran (Jilid 1). Jakarta : Salemba Baru.

Lawrence, C.A. & S. S. Block. 1968. Disinfection, steri-lizaation and preservation. Philadephia: Lea and Fe-biger.

Madduluri, Suresh. Rao, K. Babu, Sitaram B. 2013. In vitro evaluation of antibacterial activity of five inde-genous plants extract against five bacterial pathogens of human. International journal pharmacy and pharma-ceutical Sciences. 5(4), 679-684.

Naidu, A. S. & R. A. Clemens. 2000. Natural food anti-microbial systems. LCC: CRC Press.

Ngajow, M., Abidjulu J., & Kamu, V.S. 2013. Pengaruh antibakteri ekstrak kulit batang matoa (Pometia pinna-ta) terhadap bakteri Staphylococcus aureus secara in vi-tro. (Jurnal) MIPA, 2, 128-132.

Pebriana, R.B., Wardhani Kusuma, B.W., Widayanti, E., Wijayanti, N.L.S., Wijayanti, T.R., Riyanto, S., & Meiyanto, E . 2008. Pengaruh ekstrak metanolik daun

kenikir (Cosmos caudatus Kunth.) terhadap pemacuan apoptosis sel kanker payudara. Jurnal pharmacon, 9(1), 21-26.

Prihantoro, T., Indra, R., & Sumarno. 2006. Efek anti-bakteri ekstrak kulit buah delima (Punica granatum) terhadap Shigella dysentriae secara in vitro. (Jurnal). 112(3).

Putri, D.N. 2014. Uji aktivitas antibakteri ekstrak meta-nol daun kenikir (Cosmos caudatus Kunth.) terhadap bakteri Salmonella typhi. (Skripsi). Malang: Universitas Islam Negeri Maulana Malik Ibrahim.

Rasdi, N.M.H., Samah, O.A., Sule, A., & Ahmed, Q.U. 2010. Antimicrobial studies of (Cosmos caudatus Kunth.) (Composite). Journal of medicinal plants re-search, 4(8), 669-673.

Rinawati, N. D. 2011. Daya antibakteri tumbuhan ma-japahit (Crescentia cujete L.) terhadap bakteri Vibrio al-ginolyticus. (Skripsi). Surabaya: Jurusan Biologi Fakul-tas Matematika Ilmu Pengetahuan Alam Institut Tekno-logi Sepuluh November.

Warnaini, C. 2013. Uji efektivitas ekstrak kunyit sebagai antibakteri terhadap pertumbuhan bakteri Bacillus sp. dan Shigella dysentriae secara in vitro. (Jurnal): Ma-kassar: Universitas Hasanudin.

WHO. 2014. Antimicrobial resistance: global report on surveillance. 12 Februari. Web publication http : // www. who. Int / drugresistance / publications / info-graphic - antimicrobial resistance. Diakses 23 Januari 2016.

Winarsih, S., Mudjiwijono, H.E., & Diane, T.S. 2010. Efek antibakteri ekstrak etanol rimpang kunyit (Cur-cuma Dometica) terhadap pertumbuhan Shigella dy-sentriae isolat 2312-F secara in vitro. (Skripsi). Malang: Universitas Brawijaya.


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