Analisis Kloramfenikol dalam Sampel Sediaan Tetes Telinga di Kota Palembang dengan Metode Kromatografi Cair Kinerja Tinggi

Mauizatul Hasanah, Putri Wahyuni

Abstract


Determination of Chloramphenicol content in ear drops has been performed with high performance liquid chromatography (HPLC) method. The purpose of this research was to determination and know the sui-tability of Chloramphenicol contents in ear drops by reversed phase reversed phase with ultraviolet detector at a wavelength of 273.4 nm. The C18 column was used as stationary phase, methanol : water (70 : 30) v/v as mobile phase, with flow rate of 1 ml/minute. Determination of calibration curve linearity showed a linear cor-relation between the peak area and chloramphenicol concentration with concentration 5-25 μg/ml (r = 0,998). The equation of regression was y = 39020,92x + 196296, the limit of detection (LOD) value is 1,4920 μg / ml and the value of limit of quantitation (LOQ) is 4,9734 μg / ml. The precision test showed a coefficient of variation was 0.8711%. The result of determination of chloramphenicol content in ear drops were sample A = 1,17 %, sample B = 1,20 % and sample C = 1,19 %. Based on the test, samples of ear drops with contents crite-ria was fulfilled the requirements of Indonesian Pharmacopoeia fifth edition.

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References


Ahuja, S. dan Dong, M.W. 2005. Handbook of phar-maceutical analysis by HPLC. USA: Elsevier, Inc.

Alaani, H. dan Alnukkary, Y. 2016. Stability-indicating HPLC method for stimultaneous determination of Chloramphenicol, Dexamethasone Sodium Phosphate and Tetrahydrozoline Hydrochloride in opthalmic solu-tion. Advanced pharmaceutical bulletin, 6(1), 137-141.

Carolina, A. 2008. Iso farmakoterapi. Jakarta: PT. ISFI Penerbitan.

Dachriyanus. 2004. Analisa struktur senyawa organik. Universitas Andalas. Padang.

Depkes RI. 2009. Undang – undang no. 39 tahun 2009 tentang kesehatan. Jakarta: Departemen Kesehatan Republik Indonesia.

Depkes RI. 1979. Farmakope Indonesia (Edisi III). Ja-karta: Direktorat Jendral Pengawasan Obat dan Maka-nan Departemen Kesehatan Republik Indonesia.

Depkes RI. 1995. Farmakope Indonesia (Edisi IV). Ja-karta: Direktorat Jendral Pengawasan Obat dan Maka-nan Departemen Kesehatan Republik Indonesia.

FKUI. 2012. Farmakologi dan terapi. Jakarta: Badan Penerbit FKUI.

Gandjar, I.G. dan Rohman, A. 2007. Kimia farmasi ana-lisis. Yogyakarta: Pustaka Pelajar.

Gunawan, I. 1994. Metode validasi pada analisis kimia. Universitas Airlangga. Surabaya

Harmita. 2004. Petunjuk validasi metode dan cara perhitungan. Jurnal majalah ilmu kefarmasian, 1 (3), 117-135

ISO. 2014. Kloramfenikol. Volume 49. Jakarta. PT. ISFI Penerbitan.

Kazakevich, Y. dan Lobrutto, R. 2007. HPLC for phar-maceutical scientists. USA: A Jhon Willey & Sons, Inc. Publication.

Kemenkes RI. 2014. Farmakope Indonesia (Edisi V). Jakarta: Direktorat Jendral Bina Kefarmasian dan Alat Kesehatan Republik Indonesia.

Noviyanto, F., Tjiptasurasa., dan Utami, P. I. 2014. Ke-toprofen, penetapan kadarnya dalam sediaan gel den-gan metode spektrofotometri UV-Vis. Pharmacy, 01, 1-8.

Putra, E.D.L. 2004. Kromatografi Cair Kinerja Tinggi dalam bidang farmasi. Medan: Universitas Sumatera Utara.

Riyanto. 2014. Validasi dan verifikasi metode uji. Yogya-karta: Deepublish.

Sari, F. D. P. dan Utami, P. I. 2009. Penetapan kadar Kloramfenikol dalam tetes mata pada sediaan generik dan merk dengan metode Kromatografi Cair Kinerja Tinggi. Pharmacy, 06, 53-59.

Setiabudy dan Kurnadi.2001. Farmakologi dan terapi (Edisi V).Jakarta: Penerbit Gaya Baru.

Snyder, L.R. dan Kirkland, J.J. 1979. Introduction to Modern Liquid Chromatography (Edisi II). New York: A Jhon Willey & Sons, Inc. Publication.

Snyder, L.R., Kirkland, J.J., dan Dolan, J.W. 2010. Introduction to Modern Liquid Chromatography. New York: A Jhon Willey & Sons, Inc. Publication.

Sudjadi dan Rohman, A. 2012. Analisis farmasi. Yogya-karta: Pustaka Pelajar.

Suguna, P., Naidu, N.V.S., dan Sathyanarayana, B. 2014. Determination of Chloramphenicol in bulk drug and pharmaceutical dosage forms by HPLC. IOSR journal of pharmacy, 4(12), 60-70.

Tjay, T. H. dan Rahardja, K. 2007. Obat – obat penting khasiat, penggunaan, dan efek – efek sampingnya (Edisi VI). Jakarta: PT Elex Media Komputindo: hal. 65


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