Variasi Rasio Amonium dan Nitrat terhadap Perkembangan Embrio Somatik Bawang Putih (Allium sativum) secara In Vitro

Singgih Tri Wardana

Abstract


This research was aimed to conduct different NH4 + : NO3 -ratios on the development of somatic embryos of garlic (Allium sativum) by in vitro technique. This research used complete random design with four levels of NH4 + : NO3 -ratios, i.e. 1:2, 1:3, 1:4, and 1:5.The cell suspension culture used MS medium with 0,25 mg l-1 BAP. The results showed that the different NH4 + : NO3 -ratios resulted significant effect on the development of somatic  embryos ( globular, scutelar, and coleoptiler stages).1:3 ratio of NH4 + : NO3 -was optimum to improve the development of somatic embryos.


Full Text:

PDF

References


Amagase H. 2006. Clarifying the real bioactive constituents of garlic. J. Nutr. 136: 716-725.

Ayabe M. & Sumi S. 1998. Establishment of novel tissue culture method stem-disc culture and its practical

application to micropropagation of garlic (Allium sativum L.). Plant Cell Rep. 17: 773-779.

Ayabe M. & Sumi, S. 2001. A novel and efficient tissue culture method – “stem-disc dome culture†– for producing virus free garlic (Allium sativum L.). Plant Cell Rep. 20: 503-507.

Bockish T, Saranga Y, Altman. 1997. A Garlic micropropagation by somatic embryogenesis. Acta Hort. 447:

-242.

George EF. 1993. Plant propagation by tissue culture: The Technology, Part 1 2nd edition. Exegetic Limited,

London. England.

JimenezVM. 2001. Regulation of in vitro somatic embryogenesis with emphasis on the role of endogenous

hormones. Revista Brasileira de Fisiologia Vegetal. vol. 13, no. 2:196-223.

Joy IVRW., Vogel HJ &Thorpe TA. 1997. Inorganic nitrogen metabolism in embryogenic white spruce cultures

:A nitrogen 14 – 15 NMR study. J. Plant Physiol. 151 : 306 – 315.

Kim MA &Park JK. 2002. High frequency plant regeneration of garlic (Allium sativum L.) calli immobilized in

calcium alginate gel. Biotechnol.Bioprocess Eng. 7: 206- 211.

Meijer EGM &Brown DCW. 1987. Role of exogenous reduced nitrogen and sucrose in rapid high frequency

somatic embryogenesis in Medicago sativa. Plant Cell Tiss. Org.Cult. 10:11-19.

Niedz RP. 1994. Growth of embryogenic sweet orange callus on media varying in the ratio of nitrat to ammonium nitrogen. Plant Cell Tiss. Org. Cult. 39 : 1-5.

Rao KS. 1996. Embryogenesis in flowering plants: recent approaches and prospects. Journal of Biosciences.

Vol.21, No.6: 827-841.

Santanen A &Simola LK. 1994. Catabolism of putrecine and spermidine in embryogenic and nonembryogenic

callus lines of Picea abies. Physiologia Plantarum. 90 : 125 – 129.

Schwartz HF &KrishnaSM. 1999. Compendium of Onion and Garlic Diseases, the American Phytopathological

Society, St. Paul, USA.

Singh RP, Murch SJ & Saxena PK. 1999. Role of nitrogen in morphogenesis in vitro. Science Publisher Inc.

USA.

Stasolla C, Yeung EY & Thorpe TA. 2003.Somatic embryogenesis in white spruce : physiology and biochemistry. Dalam : Bhojwani, S.S. dan W.Y. Soh (eds). Agrobiotechnology and plant tissue culture. Science Publ. Inc. Enfield, NH, USA.

Thorpe TA & Stasolla C. 2001. Somatic embryogenesis. Dalam : Bhojwani, S.S. dan W.Y. Soh (eds). Current

trends in the embryology of angiosperm.Kluwer Academic Publ. Dordrecht, The Netherlands.

Wardana ST. 2004. Induksi kalus embriogenik bawang putih (Allium sativum L.) dengan rasioNH4 : NO3 yang

bervariasi. Jurnal Penelitian Sains. 16 : 88 – 96.




DOI: https://doi.org/10.56064/jps.v18i2.28

Refbacks

  • There are currently no refbacks.


   

  

 

 

Creative Commons License

Jurnal Penelitian Sains (JPS) Published by UP2M, Faculty of Mathematic and Natural Science Sriwijaya University is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

 

View My Stats