Eksplorasi Bakteri Hidrokarbonoklastik dari Rhizosfer di Lahan Tambang Minyak Rakyat, Kecamatan Babat Toman, Sumatera Selatan

Dwi Hardestyariki, Bambang Yudono, Munawar Munawar

Abstract


This study aims to obtain rhizosphere bacteria as a potential agent of bioremediation. The method of sampling the plant rhizosphere by purposive sampling. Soil samples were taken from around the rhizos-phere of plants that live in soil contaminated oil. Bacteria were isolated from the rhizosphere soil samples, then they were selected into steps : performed purification, selection I and selection II. The among bacteria are tested the synergism, then characterized and identified it’s genus. The results showed that there were as many as 34 rhizosphere bacterial isolates were collected from three different sampling locations. Identification of bacteria that have potential as a bioremediation agent. Each bacterial isolate was identified as Sporosarcina sp. A.4.10, Proteus sp. C.6.7, Actinobacillus sp. A.6.3, and Flavobacterium sp. A.5.8.

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References


Akhmaisyah. 2010. Potensi Streptomyces sp. Isolat Lok-al Dalam Mendegradasi Limbah Minyak Bumi. Skripsi. Institut Pertanian Bogor

Atlas, R.M. 1984. Petroleum Microbiology. New York: Macmillan Publishing Co.

Atlas, R.M & Bartha, R. 1992. Microbial Ecology; Fun-damentals and Applications. Third Edition. The Benja-min cummings publishing company, Inc.

Bernardet, JF and Bowman, JP. 2006. The Genus Fla-vobacterium. Journal Prokaryotes.Chapter 6.4.

Buchanan, R.E., & N.E. Gibbons. 1974. Bergeys Ma-nual of Determinative Bacteriology, 8 th ed, Williams & Wilkins, Baltimore. This volume is the standard interna-tional reference book on the classification and taxonomy of bacteria. Each major group of bacteria is described, and recognized species are characterized in detail

Gardenia, L., Koesharyani, I. Supriyadi H., Mufidah T. 2010. Aplikasi Deteksi Aeromonas hydrophila Penghasil Aerolysin dengan Menggunakan Polymerase Chain Reaction (PCR). Prosiding Forum Inovasi Teknologi Akuakultur.

Hassan, Z.H. 2006. Isolasi Lactobacillus, Bakteri Asam laktat dari feses dan organ saluran pencernaan ayam. Sem. Nas. Teknologi Peternakan dan Veteriner.

Komarawidjaja,W & Lysiastuti, E. 2009. Status Konsorsium Mikroba Lokal Pendegradasi Minyak. Jurnal Teknologi Lingkungan. Vol. 10 No.3 Hal 347- 354. ISSN 1441-318X

Macinnes, J.I, & Lally, E.T. 2006. The Prokaryotes. 3rd edition. http://www.springerreference.com

Mawazin & Subiakto, A. 2013. Keanekaragaman dan Komposisi Jenis Permudaan Alam Hutan Rawa Gam-but Bekas Tebangan di Riau. Jurnal. Vol. 1 No. 1

Munawar. 1999. Isolasi dan Uji Kemampuan Isolat Bakteri Rhizosfir dari Hutan Bakau di Cilacap dalam Mendegradasi Residu Minyak Bumi. Tesis. Program Pascasarjana ITB. Tidak dipulbikasikan

Nugroho, A. 2007. Biodegradasi Sludge Minyak Bumi Dalam Skala Mikrokosmos : Simulasi Sederhana Seba-gai Kajian Awal Bioremediasi Land Treatment. Maka-ra. Teknologi. Vol. 10. No.2.

Priyanto, B. 2010. Pengembangan Teknologi Fitoreme-diasi Untuk Menanggulangi Penyebaran Pencemaran Minyak Bumi. Laporan Akhir Program Intenstif. Badan Pengkajian dan Penerapan Teknologi

Purwaningsih. 2009. Pengujian Mikroba Sebagai Pupuk Hayati Terhadap Pertumbuhan Tanaman. Tesis. Institut Pertanian Bogor

Rao, N.S. Subra. 2007. Mikroorganisme Tanah dan Per-tumbuhan Tanaman. Ed. II. UI Press. Jakarta. 352 hlm

Silitonga, D. M, Priyani, N, dan Nurwahyuni, I. 2013. Isolasi dan Uji Potensi Isolat Bakteri Pelarut Fosfat dan Bakteri Penghasil Hormon IAA (Indole Acetic Acid) Ter-hadap Pertumbuhan Kedelai (Glycine max L.) Pada Ta-nah Kuning. Jurnal. Vol 1, No 2

Soemarno. 2010. Ekologi Tanah. http://marno.ub.ac.id

Zam,S.I. 2010. Optimasi Konsentrasi Inokulum Bakteri Hidrokarbonoklastik Pada Bioremediasi Limbah Pengilangan Minyak Bumi di Sungai Pakning. Journal of environtmental science. ISSN 1978-5283




DOI: https://doi.org/10.56064/jps.v16i3.65

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